By Richard Martin, E. Rand Sutherland
- with authoritative suggestions from 15 professional foreign specialists within the parts of pulmonology, microbiology, immunology, hypersensitive reaction, and pediatrics
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Additional info for Asthma and Infections, Volume 238 (Lung Biology in Health and Disease)
The presence of abundant normal microbial flora within the oral cavity poses difficulties for obtaining uncontaminated specimens. The question then becomes: are the organisms isolated and identified by the laboratory a cause of the infectious process or are they merely colonizing the oropharynx? However, the presence of some microorganisms such as Legionella pneumophila, Mycobacterium tuberculosis, Pneumocystis carinii, most viruses, and pathogenic dimorphic fungi is often considered proof that these are the etiological agents of an ongoing disease.
Cell cultures of primary, diploid, and heteroploid cells are used in various combinations to support the growth of common respiratory viruses. Most laboratories can now purchase cell lines that will support the growth of the viruses that may be of interest. Cell culture is the method by which all other methods have been compared, including molecular diagnostic methods. The advantages of cell culture is the broad range of viruses that can be detected, increased sensitivity over the rapid antigen methods, and the ability to isolate the virus for further study if needed.
Boivin G, Cote S, Dery P, et al. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J Clin Microbiol 2004; 42(1):45–51. 48. Brunstein J, Thomas E. Direct screening of clinical specimens for multiple respiratory pathogens using the Genaco Respiratory Panels 1 and 2. Diagn Mol Pathol 2006; 15(3):169–173. 49. Coiras MT, Aguilar JC, Garcia ML, et al. Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays.
Asthma and Infections, Volume 238 (Lung Biology in Health and Disease) by Richard Martin, E. Rand Sutherland