By L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)
Flow cytometry has speedily developed right into a process for quick research of DNA content material, mobile marker expression and digital sorting of cells of curiosity for additional investigations. stream cytometers are being widely used for tracking of mobile DNA content material, phenotype expression, drug delivery, calcium flux, proliferation and apoptosis. Phenotypic research of marker expression in leukemic cells has develop into a huge software for diagnostic and healing tracking of sufferers. contemporary experiences have explored using movement cytometry for tracking hormone receptor expression in human good tumors and for experiences in human genomics. Contributions within the present quantity are in line with shows made on the First Indo-US workshop on circulate Cytometry during which specialists from united states, united kingdom and India mentioned functions of circulate cytometry in organic and clinical learn. This publication can be of curiosity to put up graduates and researchers within the fields of pathology, cytology, telephone biology and molecular biology.
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Extra resources for Advanced Flow Cytometry: Applications in Biological Research
The interpretation may be difficult at times due to change in the phenotype of the cells. Cells with similar surface phenotype but synthesizing different cytokines and having different functional characteristics can be analyzed with this technique. Key words: Cytokines, Flowcytometry, Intra-cellular 1. Introduction Flowcytometry is a very refined and advanced technique in the field of immunology. Although, surface markers on some of the cells correlate with their function, it may not always be possible to infer the function of a cell by surface staining as phenotypically similar cells may perform different functions in vivo due to the expression of a different set of proteins intracellularly.
Acta Cytol 41: 123-143. 5. Kawasaki M, Sasaki K, Satoh T, Kurose A, Kamada T, Furuya T, Murakami T, Todoroki T (1997). Laser scanning cytometry (LSC) allows detailed analysis of the cell cycle in PI stained human fibroblasts (TIG7). Cell Prolif 30 (3-4): 139-147. 6. Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schram! P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP (1998). Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 4: 844-847. 7.
Put drops of fixed-cell suspensions in duplicate on the cell array glass slide with a specially designed chip to reduce variability among samples [10, 14]. ~I . ' .. ~" -1C PI Integral Figure 2. DNA cytograms indicate the cell cycle position of each cell. Most of cells are present in the mitotic phase or post-mitotic phase. Abscissa: PI fluorescence value representing DNA content, ordinate: peak fluorescence value representing the status of chromatin condensation. 44 (A) (B) Figure 3. Changes in fluorescence images of cyclin expression during the cell cycle.
Advanced Flow Cytometry: Applications in Biological Research by L. Scott Cram (auth.), R. C. Sobti, Awtar Krishan (eds.)